COMPARATIVE STUDIES OF MAGNESIUM CONTENT IN PANCREATIC ISLET AND IMMUNE SYSTEM CELLS
DOI:
https://doi.org/10.32782/2450-8640.2023.2.1Keywords:
magnesium, functional state, insular apparatus, thymus, blood lymphocytes.Abstract
The work is devoted to conducting comparative studies of magnesium content in pancreatic β-cells, thymic epithelial cells and blood lymphocytes of animals during activation, suppression and blocking of the secretory function of the pancreas. The relevance of studies of the content of intracellular magnesium is due to the importance of this metal for the functioning of the insular apparatus and the immune system, as well as the involvement of the latter in the mechanisms of the development of insulin-dependent diabetes mellitus. The development in our laboratory of the cytochemical reaction of lumomagnesone to magnesium in cells of the blood, pancreas and thymus allowed us to conduct such studies. Experiments were conducted on outbred mice and rats aged 6 months. The state of suppression of the secretory function of β-cells of pancreatic islets was obtained by administering atropine, adrenaline, prednisone and acute starvation to the animals. An increase in the secretory activity of these cells was caused by injections of glucose, pilocarpine, and cholecystokinin. Experimental data were processed using Student’s t-test. The Pearson correlation coefficient (r) was calculated to assess the degree of relationship between changes in the studied indicators. The state of hypofunction of the islet apparatus of the pancreas was modeled by introducing the diabetogenic substance streptozotocin to the animals. It was established that suppression of the secretory activity of insulin-producing cells caused an increase in magnesium content by 17% (Р < 0,05) – 39% (Р < 0,001), and activation of this function, on the contrary, decreased its content by 25% (Р < 0,05) – 37% (Р < 0,001) in pancreatic β cells, epithelial cells of the thymus, blood lymphocytes of mice and rats. Blocking the function of the insular apparatus after streptozotocin injection led to the development of pronounced metal deficiency in the studied cells, which varied between 44–54% (Р < 0,001). In all cases, there was a positive correlation of changes in magnesium content in islet β-cells, thymus cells, and blood lymphocytes of experimental animals, which indicates the existence of close functional connections between the insular apparatus and the immune system.
References
Вміст магнію в панкреатичних клітинах β, ТЕК і лімфоцитах крові мишей і щурів збільшувався на 17% (Р
The assessment of intracellular magnesium: different strategies to answer different questions / G. Picone et al. Magnes Res. 2020. Vol. 33. № 1. Р. 1–11.
Lima F.D.S, Fock R.A.A. Review of the аction of magnesium on several processes involved in the modulation of hematopoiesis. Int. J. Mol. Sci. 2020. Vol. 21. Issue 19. Р. 70–84.
Role of magnesium in insulin action, diabetes and cardiometabolic syndrome X / M. Barbagallo et al. Mol. Aspects Med. 2003. Vol. 24. Issue 1–3. P. 39–52.
Participation of magnesium in the secretion and signaling pathways of insulin: an updated review / S.R. de Sousa Melo et al. Biol. Trace Elem. Res. 2022. Vol. 200. Issue 8. Р. 3545–3553.
Magnesium in man: implication for health and disease / J.H. De Baaij et al. Physiol. Rev. 2015. Vol. 95. P. 1–46.
The role of magnesium in the pathogenesis of metabolic disorders / M. Pelczyńska et al. Nutrients. 2022. Vol. 14. Issue 9. Р. 1711–1714.
Possible roles of magnesium on the immune system / M. Tam et al. Eur. J. Clin. Nutr. 2003. Vol. 57. Issue 10. P. 1193–1197.
Role of magnesium in the intensive care unit and immunomodulation: a literature review / F. Saglietti et al. Vaccines (Basel). 2023. Vol. 11. Issue 6. Р. 1112–1122.
Batar P.K. Study of serum magnesium level in diabetes mellitus and it’s correlation with micro and macro complications. J. Assoc. Physicians India. 2022. Vol. 70. Issue 4. Р. 11–12.
Vitamin B12, folic acid, vitamin D, iron, ferritin, magnesium, and HbA1c levels in patients with diabetes mellitus and dental prosthesis / S. Ciftel et al. Eur. Rev. Med. Pharmacol. Sci. 2022. Vol. 26. Issue 19. Р. 7135–7144.
Total plasma magnesium, zinc, copper and selenium concentrations in type-I and type-II diabetes / A.I.S. Sobczak et al. Biometals. 2019. Vol. 32. Issue 1. Р. 123–138.
Van Laecke S. Hypomagnesemia and hypermagnesemia. Acta Clin. Belg. 2019. Vol. 74. Issue 1. Р. 41–47.
De Valk H.W. Magnesium in diabetes mellitus. Neth. J. Med. 1999. Vol. 54. P. 39–52.
Lernmark A., Torre D.Le. Immunology of β-cell destruction. Islets of Langergans. 2015. Vol. 7. P. 1047–1080.
The heterogeneous pathogenesis of type 1 diabetes mellitus / J. Ilonen et al. Nat. Rev. Endocrinol. 2019. Vol. 15. Issue 11. Р. 635–650.
Type 1 diabetes mellitus as a disease of the β-cell (do not blame the immune system?) / B.O. Roep et al. Nat. Rev. Endocrinol. 2021. Vol. 17. Issue 3. Р. 150–161.
Importance of a thymus dysfunction in the pathophysiology of type 1 diabetes / V. Geenene et al. Kev. Med. Liege. 2005. Vol. 60. Issue 5–6. P. 291–296.
