IMPROVEMENT OF LACCASE-BASED AMPEROMETRIC BIOSENSOR WITH TITANIUM DIOXIDE NANOPARTICLES
DOI:
https://doi.org/10.32782/2450-8640.2021.1.4Keywords:
TiO2, Nafion®, laccase, ABTS, amperometric biosensorAbstract
The development of new approaches for monitoring of the dangerous substances in environment is a topical present problem to improve human life quality. In this point of view, electrochemical biosensors have received increased attention due to their high specificity, sensitivity, reliability, portability and simplicity in operation. Nowadays, there is a rapid growth in the use of semiconductor nanoparticles (NPs) in fabricating electrochemical sensors, stimulated with the unique properties of such nanomaterials as large surface area and good biocompatibility. The NPs-modified laccase based biosensors are very promising in quantifying phenolic compounds with good precision and accuracy. Here, we described construction of biosensor for phenols analysis based on laccase combined with commercial TiO2 nanoparticles incorporated in Nafion® polymer. The constructed bioelectrodes have demonstrated improving the operational parameters compare with the bioelectrodes without usage of TiO2 that make them more promising for the phenols analysis in the real samples of wastewater and ground water.
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