EVALUATION OF THE CONTENT OF SOME BIOLOGICALLY ACTIVE SUBSTANCES IN BERRIES OF JUNIPERUS COMMUNIS PLANTS FROM NATURAL POPULATIONS OF THE UKRAINIAN CARPATHIANS
DOI:
https://doi.org/10.32782/2450-8640.2023.2.3Keywords:
Juniperus communis, berries, extracts, biologically active substances, seasonal variability, population variability.Abstract
Juniperus communis berries are an important medicinal plant raw material due to the high content of biologically important compounds that determine its therapeutic effect, as well as the pharmacological activity of preparations, extracts, biologically active supplements (BASs), phytoteas made on its basis. The qualitative and quantitative composition of medicinal raw materials can change depending on the genetic structure of the population and the soil and climatic conditions of plant growth, that’s why the content of pharmacologically important substances in each collection must be constantly monitored. The content of some biologically active substances in the berries of J. communis plants, which grow in the territory of the Lviv region in five different locations of the lower mountain belt of the Ukrainian Carpathians, was evaluated. A comparative analysis of the total content of polyphenols, ascorbic acid, and carotene in the fruits of J. communis harvested in 2019–2023 was carried out. It was found that the total content of polyphenols and ascorbic acid in the berries of J. communis has seasonal variability. It was found that the total content of polyphenols (Folin – Chocalteu method, calculated on gallic acid (GA)), extracted with 70% ethanol from crushed berries, vary in limits from 200 ± 10 to 340 ± 20 mg GA/g absolutely dry mass (ADM). The content of ascorbic acid in juniper berries varies in a narrower range of values from 1,11 ± 0,05 to 1,48 ± 0,07 mg/g ADM. The carotene content in the 2019–2023 collections of J. communis berries is insignificant, on average it is 2,36 ± 0,11 mg/g ADM, seasonal and population variability is not observed. It was found that the berries of J. communis plants, which grow in the territory of the Lviv region in different locations of the Ukrainian Carpathians, differ in the content of polyphenols by 1,25–1,7 times and ascorbic acid by 1,33 times, but not in carotene.
References
Лікарські рослини : енциклопедичний довідник / за ред. А.М. Гродзінського. Київ : Українська енциклопедія ім. М.П. Бажана, Український виробничо-комерційний центр «Олімп», 1992. С. 490–491.
Біологічно активні речовини і лікувальні препарати з Ялівцю звичайного / О.Г. Малик та ін. Біологія тварин. 2010. Т. 12 (1). С. 37–43.
Фармакологічні властивості Ялівцю звичайного (Juniperus communis) та екологічні особливості його поширення на Дрогобиччині / С.Я. Волошанська та ін. Науковий вісник Національного лісотехнічного університету України. 2014. Вип. 24.4.
С. 179–184. URL: https://nv.nltu.edu.ua/Archive/2014/24_4/179_Vol.pdf.
Adams R.P. Junipers of the World: The Genus Juniperus. 4’th ed. Bloomington, IN, USA : Trafford Publishing Company, 2014. 436 p. URL: http://surl.li/mlami.
Potential of Juniperus communis L as a nutraceutical in human and veterinary medicine / R. Raina et al. Heliyon. 2019. Vol. 5 (8) : e02376. DOI: 10.1016/j.heliyon.2019.e02376.
Біологічна дія та застосування фітопрепаратів на основі ялівцю звичайного. Biological effects and applicationon on the basis of herbal Juniper usual. / М. Козар та ін. Acta Carpathica. 2013. № 10. С. 97–102. URL: http://surl.li/mkoue.
Euforgen. Juniperus communis. URL: https://www.euforgen.org/species/juniperuscommunis.
Preparation and study of Juniper berry and seed extracts / M. Torosyan et al. Proceedings of the YSU B: Chemical and Biological Sciences. 2023. Vol. 57. P. 177–181. DOI: 10.46991/PYSU:B/2023.57.2.177.
Antioxidant and antimicrobial activities of branches extracts of five Juniperus species from Turkey. Pharm Biol / M.F. Taviano et al. 2011. Vol. 49 (10). P. 1014–1022. DOI: 10.3109/13880209.2011.
Chemical composition and antioxidant properties of Juniper berry (Juniperus communis L.) essential oil. Action of the essential oil on the antioxidant protection of Saccharomyces cerevisiae model organism / M. Höferl et al. Antioxidants. 2014. Vol. 3 (1). P. 81–98.
https://doi.org/10.3390/antiox3010081.
Comparative analysis of flavonoid profile, antioxidant and antimicrobial activity of the berries of Juniperus communis L. var. communis and Juniperus communis L. var. saxatilis Pall. from Turkey / N. Miceli et al. J Agric Food Chem. 2009. Vol. 57 (15). P. 6570–6577. DOI: 10.1021/jf9012295. PMID: 19580284.
Assessment of the methods for obtaining alcoholic exstracts from Juniper berry (Juniperus communis L.) / H.M. Klepach et al. Acta Carpathica. 2017. 28. P. 63–72. URL: https://journals.dspu.in.ua/index.php/actacarpathica/issue/view/27.
Biologically active properties of the ethanol and aqueous extracts from the needles / H. Klepach et al. Juniperus communis / PNAP (Scientific Jornal of Polonia University). 2019. Vol. 34 (3). P. 104–112. DOI: http://dx.doi.org/10.23856/3413.
Modulatory effect of standardised amentoflavone isolated from Juniperus communis L. agianst Freund’s adjuvant induced arthritis in rats (histopathological and X Ray anaysis) / S. Bais et al. Biomed Pharmacother. 2017. Vol. 86. P. 381–392. DOI: 10.1016/j.biopha.2016.12.027.
Antioxidant and Hepatoprotective Potential of Phenol-Rich Fraction of Juniperus communis Linn. Leaves / A. Ved et al. Pharmacogn Mag. 2017. Vol. 13 (49). P. 108–113. DOI: 10.4103/0973-1296.197648.
Tyrosinase inhibitory flavonoid from Juniperus communis fruits / J. Jegal et al. Biosci Biotechnol Biochem. 2016. Vol. 80 (12). P. 2311–2317. doi.org/10.1080/09168451.2016.1217146.
Analysis of total phenols and other oxidations substractes and antioxidans by means of Folin-Ciocalteu reagent / V.L. Singleton et al. Methods in enzymology. 1999. Vol. 299. P. 152–178. DOI: https://doi.org/10.1016/S0076-6879(99)99017-1.
Davies S.H.R., Masten S.J. Spectrophotometric method for ascorbic acid using dichlorophenolindophenol: elimination of the interference due to iron. Anal. Chim. Acta. 1991. Vol. 248 (1). P. 225. URL: https://zlibrary.to/dl/analytica-chimica-acta-1991-vol-248-index.
Сучасні методи біохімічного аналізу рослин : навчальний посібник / Л.В. Шупранова та ін. Дніпро : Вид-во ДНУ, 2011. 80 с.
Seasonal variability of Juniperus communis L. berry ethanol extracts : 2. In vitro ferric reducing ability of plasma (FRAP) assay / J. Fejér et al. Molecules. 2022. Vol. 27 (24). P. 9027. DOI: 10.3390/molecules27249027. PMID: 36558161; PMCID: PMC9787287.
Антиоксидантна дія екстрактів окремих лікарських рослин / С.С. Монастирська та ін. Вісник Львівського університету. Серія «Біологічна». 2016. Вип. 73. С. 409–412. URL: http://surl.li/mlvij.
